Plasmid-free expression platforms
Chromosomally-directed expression of antigens and proteins in general is an excellent alternative to overcome problems related to replicative plasmids (instability, supplementation of culture media with antibiotics for avoiding plasmid loss, fitness cost associated to plasmid replicaron…etc.).
Chromosomally integrated expression cassettes might not only be used as novel platforms for recombinant protein production but also represent an excellent tool for developing novel antigen-delivery systems and multivalent vaccine candidates.
Following a Synthetic biology approach, RECOMBINA offers its own collection of biological parts like promoters, RBS, fusion proteins and bacterial-optimized or viral antigens for developing customized bacterial strains capable of expressing heterologous antigens.
Such a technique prevents legal problems associated to IP-protected plasmids and expression systems.